Nucleotide sequence at the 5' extremity of turnip yellow mosaic virus genome RNA
نویسندگان
چکیده
منابع مشابه
Replication and packaging of Turnip yellow mosaic virus RNA containing Flock house virus RNA1 sequence
Turnip yellow mosaic virus (TYMV) is a spherical plant virus that has a single 6.3 kb positive strand RNA as a genome. In this study, RNA1 sequence of Flock house virus (FHV) was inserted into the TYMV genome to test whether TYMV can accommodate and express another viral entity. In the resulting construct, designated TY-FHV, the FHV RNA1 sequence was expressed as a TYMV subgenomic RNA. Northern...
متن کاملReplication and encapsidation of recombinant Turnip yellow mosaic virus RNA.
Turnip yellow mosaic virus (TYMV) is a positive strand RNA virus that infects mainly Cruciferae plants. In this study, the TYMV genome was modified by inserting an extra subgenomic RNA promoter and a multiple cloning site. This modified TYMV was introduced into Nicotiana benthamiana using a Agrobacterium-mediated T-DNA transfer system (agroinfiltration). When a gene encoding beta-glucuronidase ...
متن کاملCis-preferential replication of the turnip yellow mosaic virus RNA genome.
The largest open reading frame of the turnip yellow mosaic virus RNA genome encodes a 206-kDa protein that is cleaved to yield N-terminal 150-kDa (p150) and C-terminal 70-kDa (p70) proteins. Using a genomic cDNA clone capable of generating infectious transcripts in vitro, we have introduced substitution, frameshift, and in-frame deletion mutations into the regions encoding both proteins. None o...
متن کاملThe 5'-proximal hairpin of turnip yellow mosaic virus RNA: its role in translation and encapsidation.
The RNA genome of turnip yellow mosaic virus (TYMV) consists of more than 6,000 nucleotides. During a study of the roles of the two hairpins located in its 90-nucleotide 5' untranslated region, it was observed that stabilization of the 5'-proximal hairpin leads to a delay in the development of symptoms on plants. This delay in symptom development for both locally and systemically infected leave...
متن کاملEquilibrium sedimentation of turnip yellow mosaic virus.
Sedimentation equilibrium was achieved with turnip yellow mosaic virus at low speeds (600 rpm) in a magnetic ultracentrifuge. The experiments were carried out in the newly installed constant-speed rotor, equipped with automatic control and an electromagnetic drive. A particle mass of 5.55 x 10(6) daltons was calculated for the virus at vanishing concentrations, in essential agreement with the e...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1978
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.75.7.3168